NTD – RRM Stacking

We are pleased to announce that our latest TDP-43 study has been published: Identifying interactions between TDP-43’s N-terminal and RNA-binding domains. In this Protein Science publication, we present 2D NMR experiments showing allosteric shifts in the RNA‑binding surfaces of the RRMs in the presence of NTD. Carr-Purcell-Meiboom-Gill (CPMG)-NMR results also support NTD interaction with the RRMs and protein‑protein docking presents several possible sites of interaction. Further, we show that NTD modulates affinity for RNA, supporting regulatory role for NTD in TDP‑43 – RNA interactions.

Congrats to all!